Selected publications:



   Antibiotic-tolerant persister cells


   Windels E.M.*, Michiels J.E.*, Fauvart M., Wenseleers T., Van den Bergh B., Michiels J. (2019) Bacterial persistence promotes

   the evolution of antibiotic resistance by increasing survival and mutation rates.

   ISME J. doi:10.1038/s41396-019-0344-9 -- PubMed -- Publisher -- PDF --

   

   Wilmaerts D., Bayoumi M., Dewachter L., Knapen W., Mika J.T., Hofkens J., Dedecker P., Maglia G., Verstraeten N., Michiels J.   (2018)

   The persistence-inducing toxin HokB forms dynamic pores that cause ATP leakage. mBio. 9:e00744-18 -- PubMed -- Publisher -- PDF --

   Fauvart M., Van den Bergh B., Michiels J. (2018) Stabbed while sleeping: synthetic retinoid antibiotics kill bacterial persister cells. Mol.

   Cell. 70:763-764 -- PubMed -- Publisher -- PDF --

   Defraine V., Fauvart M., Michiels J. (2018) Fighting bacterial persistence: current and emerging anti-persister strategies and therapeutics.

   Drug. Resist. Updat. 38:12-26 -- PubMed -- Publisher -- PDF --

   Ghequire M.G.K., Swings T., Michiels J., Buchanan S.K., De Mot R. (2018) Hitting with a BAM: Selective Killing by Lectin-Like

   Bacteriocins.  mBio. doi: 10.1128/mBio.02138-17 -- PubMed -- Publisher -- PDF --

    Defraine V., Liebens V., Loos E., Swings T., Weytjens B., Fierro C., Marchal K., Sharkey L., O’Neill A.J., Corbau R., Marchand A.,

    Chaltin   P., Fauvart M., Michiels J. (2018) 1-((2,4-dichlorophenethyl)amino)-3-phenoxypropan-2-ol kills Pseudomonas aeruginosa

    through   extensive membrane damage. Front. Microbiol. -- PubMed -- PDF --

    Defraine V., Verstraete L., Van Bambeke F., Anantharajah A., Townsend E.M., Ramage G., Corbau R., Marchand A., Chaltin P.,

    Fauvart M.,  Michiels J. (2017) Antibacterial Activity of 1-[(2,4-Dichlorophenethyl)amino]-3-Phenoxypropan-2-ol against

   Antibiotic-Resistant  Strains of Diverse Bacterial Pathogens, Biofilms and in Pre-clinical Infection Models. Front. Microbiol.

   8:2585 -- PubMed -- PDF --

   Liebens V.*, Defraine V.*, Knapen W., Swings T., Beullens S., Corbau R., Marchand A., Chaltin P., Fauvart M., Michiels J. (2017)  

   Identification of 1-((2,4-dichlorophenethyl)amino)-3-phenoxypropan-2-ol, a novel antibacterial compound active against

   persisters of

   Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 61:e00836-17 -- PubMed -- PDF --

   Van den Bergh B., Fauvart M., Michiels J. (2017) Formation, physiology, ecology, evolution and clinical importance of bacterial  

   persisters.

   FEMS Microbiol. Rev. 41:219-251 -- PubMed -- PDF --

   Gerits E., Defraine V., Vandamme K., De Cremer K., De Brucker K., Thevissen K., Cammue B.P., Beullens S., Fauvart M.,

   Verstraeten N.,   Michiels J. (2017) Repurposing toremifene for the treatment of oral bacterial infections. Antimicrob.

   Agent Chemother.

   DOI: 10.1128/AAC.01846-16 -- PubMed -- PDF --

   Michiels J.E., Van den Bergh B., Verstraeten N., Michiels J. (2016) Molecular mechanisms and clinical implications of bacterial

   persistence.

   Drug. Resist. Updat. 29:76-89 -- PubMed -- PDF --

   Van den Bergh B., Michiels J.E., Fauvart M., Michiels J. (2016) Should we develop screens for multi-drug antibiotic tolerance?

   Expert Rev.  Anti-Infect. Ther. doi:10.1080/14787210.2016.1194754 -- PubMed -- PDF --

   Michiels J.E., Van den Bergh B., Verstraeten N., Fauvart M., Michiels J. (2016) In vitro emergence of high persistence upon

   periodic

   aminoglycoside challenge in the ESKAPE pathogens. Antimicrob. Agents Chemother. doi: 10.1128/AAC.00757-16 -- PubMed --

   PDF --

   Defraine V., Schuermans J., Grymonprez B., Govers S.K., Aertsen A., Fauvart M., Michiels J., Lavigne R., Briers Y. (2016)

   Efficacy of

   Artilysin Art-175 against Resistant and Persistent Acinetobacter baumannii . Antimicrob Agents Chemother. 23:3480-3488

   -- PubMed -- PDF --

  Gerits E., Kucharíková S., Van Dijck P., Erdtmann M., Krona A., Lövenklev M., Fröhlich M., Dovgan B., Impellizzeri F., Braem A.,

  VleugelsJ.,

  Robijns S.C., Steenackers H.P., Vanderleyden J., De Brucker K., Thevissen K., Cammue B.P., Fauvart M., Verstraeten N.,

  Michiels J. (2016)

  Antibacterial activity of a new broad-spectrum antibiotic covalently bound to titanium surfaces. J. Orthop. Res.

  doi:10.1002/jor.23238 --

  PubMed -- PDF --

  Gerits E., Blommaert E., Lippell A., O'Neill A.J., Weytjens B., De Maeyer D., Fierro A.C., Marchal K., Marchand A., Chaltin P.,

  Spincemaille P., De Brucker K., Thevissen K., Cammue B.P., Swings T., Liebens V., Fauvart M., Verstraeten N., Michiels J. (2016) Elucidation of the Mode of Action of a New Antibacterial Compound Active against Staphylococcus aureus and Pseudomonas aeruginosa. PLoS One 11(5):e0155139 -- PubMed -- PDF --

Van den Bergh B., Michiels J.E., Wenseleers T., Windels E.M., Vanden Boer P., Kestemont D., De Meester L., Verstrepen K.J., Verstraeten N., Fauvart M. & Michiels J. (2016) Frequency of antibiotic application drives rapid evolutionary adaptation of Escherichia coli persistence. Nat. Microbiol. doi: 10.1038/nmicrobiol.2016.20 -- PubMed -- Publisher -- PDF --

Verstraeten N., Knapen W.J., Kint C.I., Liebens V., Van den Bergh B., Dewachter L., Michiels J.E., Fu Q., David C.C., Fierro A.C., Marchal K., Beirlant J., Versées W., Hofkens J., Jansen M., Fauvart M., Michiels J. (2015) Obg and membrane depolarization are part of a microbial bet-hedging strategy that leads to antibiotic tolerance. Mol. Cell. doi: 10.1016/j.molcel.2015.05.011 -- PubMed -- PDF -- Preview in Molecular Cell -- Highlight in Nature Reviews Microbiology -- Recommendation in F1000Prime (PDF) -- Microreview in Microbial Cell --

Stepanyan K., Wenseleers T., Duéñez-Guzmán E.A., Muratori F., Van den Bergh B., Verstraeten N., De Meester L., Verstrepen K.J., Fauvart M., Michiels J. (2015) Fitness trade-offs explain low levels of persister cells in the opportunistic pathogen Pseudomonas aeruginosa. Mol. Ecol. 24:1572-1583 -- PubMed -- PDF --

Liebens V., Gerits E., Knapen W.J., Swings T., Beullens S., Steenackers H.P., Robijns S., Lippell A., O'Neill A.J., Veber M., Fröhlich M., Krona A., Lövenklev M., Corbau R., Marchand A., Chaltin P., De Brucker K., Thevissen K., Cammue B.P., Fauvart M., Verstraeten N., Michiels J. (2014) Identification and characterization of an anti-pseudomonal dichlorocarbazol derivative displaying anti-biofilm activity. Bioorg. Med. Chem. Lett. 24(23):5404-5408 -- PubMed -- PDF --

Liebens V., Defraine V., Van der Leyden A., De Groote V.N., Fierro C., Beullens S., Verstraeten N., Kint C., Jans A., Frangipani E., Visca P., Marchal K., Versées W., Fauvart M., Michiels J. (2014) A putative de-N-acetylase of the PIG-L superfamily affects fluoroquinolone tolerance in Pseudomonas aeruginosa. Pathog. Dis. 71:39-54 -- PubMed -- PDF --

Kint C.I., Verstraeten N., Fauvart M., Michiels J. (2012) New-found fundamentals of bacterial persistence. Trends Microbiol. 20:577-585 -- PubMed -- PDF --

Fauvart M., De Groote V.N., Michiels J. (2011) Role of persister cells in chronic infections: clinical relevance and perspectives on anti-persister therapies. J. Med. Microbiol. 60:699-709 -- PubMed -- PDF -- LabNews feature  --

De Groote V.N., Fauvart M., Kint C.I., Verstraeten N., Jans A., Cornelis P., Michiels J. (2011) Pseudomonas aeruginosa fosfomycin resistance mechanisms affect non-inherited fluoroquinolone tolerance. J. Med. Microbiol. 60:329-336 -- PubMed -- PDF -- JMM Editorial -- SGM Press Release  -- De Standaard (piece in Belgian newspaper)  -- NRC Handelsblad (piece in Dutch newspaper)  --

De Groote V.N., Verstraeten, N., Fauvart, M., Kint, C.I., Verbeeck, A.M., Beullens, S., Cornelis, P., Michiels, J. (2009) Novel persistence genes in Pseudomonas aeruginosaidentified by high-throughput screening. FEMS Microbiol. Lett. 297:73-79 -- PubMed -- PDF -- Faculty of 1000 evaluation  --


Universally conserved prokaryotic GTPases

Dewachter L., Verstraeten N., Fauvart M., Michiels J. (2018) An integrative view of cell cycle control in Escherichia coli. FEMS Microbiol. Rev. 42(2):116–136 -- PubMed -- PDF -- Listed as 'Top 10 FEMS Microbiology Reviews articles making an impact' --

Dewachter L., Verstraeten N., Jennes M., Verbeelen T., Biboy J., Monteyne D., Pérez-Morga D., Verstrepen K.J., Vollmer W., Fauvart M., Michiels J. (2017) A mutant isoform of ObgE causes cell death by interfering with cell division. Front. Microbiol. 8:1193 -- PubMed -- PDF --

Dewachter L., Herpels P., Verstraeten N., Fauvart M., Michiels J. (2016) Reactive oxygen species do not contribute to ObgE*-mediated programmed cell death. Sci. Rep. 6:33723 -- PubMed -- PDF --

Dewachter L., Verstraeten N., Monteyne D., Kint C.I., Versées W., Pérez-Morga D., Michiels J., Fauvart M. (2015) A single-amino acid substitution in Obg activates a new programmed cell death pathway in Escherichia coli. mBio 6:e01935-15. PubMed -- PDF -- cover of the December issue -- Microreview in Microbial Cell --

Kint C.I., Verstraeten N., Hofkens J., Fauvart M., Michiels J. (2014) Bacterial Obg proteins: GTPases at the nexus of protein and DNA synthesis. Crit. Rev. Microbiol. 40:207-224 -- PubMed -- PDF --

Kint C.I., Verstraeten N., Wens I., Liebens V.R., Hofkens J., Versées W., Fauvart M., Michiels J. (2012) The Escherichia coli GTPase ObgE modulates hydroxyl radical levels in response to DNA replication fork arrest. FEBS J. 279:3692-3704 -- PubMed -- PDF --

Verstraeten N., Fauvart M., Versées W., Michiels J. (2011) The universally conserved prokaryotic GTPases. Microbiol. Mol. Biol. Rev. 75:507-542 -- PubMed -- PDF --


Artificial genetic variation for stress tolerance in bacteria

Van den Bergh B., Swings T., Fauvart M., Michiels J. (2018) Experimental design, population dynamics, and diversity in microbial experimental evolution. Microbiol. Mol. Biol. Rev. 82:e00008-18. doi: 10.1128/MMBR.00008-18 -- Publisher -- PDF --

Swings T., Marciano D.C., Atri B., Bosserman R.E., Wang C., Leysen M., Bonte C., Schalck T., Furey I., Van den Bergh B., Verstraeten N., Christie P.J., Herman C., Lichtarge O., Michiels J. (2018) CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing. Nat. Commun. 9:2231. doi: 10.1038/s41467-018-04651-5. -- PubMed -- Publisher -- PDF --

Swings T., Weytjens B., Schalck T., Bonte C., Verstraeten N., Michiels J., Marchal K. (2017) Network-based identification of adaptive pathways in evolved ethanol-tolerant bacterial populations. Mol. Biol. Evol. doi:10.1093/molbev/msx228 -- Publisher -- PDF --

Swings T., Van den Bergh B., Wuyts S., Oeyen E., Voordeckers K., Verstrepen K.J., Fauvart M., Verstraeten N., Michiels J. (2017) Adaptive tuning of mutation rates allows fast response to lethal stress in Escherichia coli. eLife. 6:e22939 -- PubMed -- PDF --


Biological nitrogen fixation in the legume - Rhizobium symbiosis

Gao S., Romdhane S.B., Beullens S., Kaever V., Lambrichts I., Fauvart M., Michiels J. (2014) Genomic analysis of cyclic-di-GMP-related genes in rhizobial type strains and functional analysis in Rhizobium etli. Appl. Microbiol. Biotechnol. 98:4589-4602 -- PubMed -- PDF

Jans A., Vercruysse M., Gao S., Engelen K., Lambrichts I., Fauvart M., Michiels J. (2013) Canonical and non-canonical EcfG sigma factors control the general stress response in Rhizobium etli. MicrobiologyOpen. doi: 10.1002/mbo3.137 -- Publisher -- PDF

Vercruysse M., Fauvart M., Beullens S., Braeken K., Cloots L., Engelen K., Marchal K., Michiels J. (2011) A comparative transcriptome analysis of Rhizobium etli bacteroids: specific gene expression during symbiotic non-growth. Mol. Plant-Microbe Interact. 24:1553-1561 -- PubMed -- PDF

Fauvart M., Sánchez-Rodríguez A., Beullens S., Marchal K., Michiels J. (2011) Genome sequence of Rhizobium etli CNPAF512, a nitrogen-fixing symbiont isolated from bean root nodules in Brazil. J. Bacteriol. 193:3158-3159 -- PubMed -- PDF

Vercruysse M., Fauvart M., Jans A., Beullens S., Braeken K., Cloots L., Engelen K., Marchal K., Michiels J. (2011) Stress response regulators identified through genome-wide transcriptome analysis of the (p)ppGpp-dependent response in Rhizobium etli. Genome Biol. 12:R17 -- PubMed -- PDF

Vercruysse M., Fauvart M., Cloots L., Engelen K., Thijs I.M., Marchal K., Michiels J. (2010) Genome-wide detection of predicted non-coding RNAs in Rhizobium etli expressed during free-living and host-associated growth using a high-resolution tiling array. BMC Genomics. 11:53 -- PubMed -- PDF 

Vos K., Braeken K., Fauvart M., Ndayizeye M., Verhaert J., Zachurzok S., Lambrichts I., Michiels J. (2007) The Rhizobium etli opt operon is required for symbiosis and stress resistance. Environ. Microbiol. 9:1665-1674 -- PubMed -- PDF

Daniels R., Reynaert S., Hoekstra H., Verreth C., Janssens J., Braeken K., Fauvart M., Beullens S., Heusdens C., Lambrichts I., De Vos D.E., Vanderleyden J., Vermant J., Michiels J. (2006) Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli. Proc. Natl. Acad. Sci. USA. 103:14965-14970 -- PubMed -- PDF

Braeken K., Moris M., Daniels R., Vanderleyden J., Michiels J. (2006) New horizons for (p)ppGpp in bacterial and plant physiology. Trends Microbiol. 14: 45-54 -- PubMed -- PDF

Moris M., Braeken K., Schoeters E., Verreth C., Beullens S., Vanderleyden J., Michiels J. (2005) Effective symbiosis between Rhizobium etli and Phaseolus vulgaris requires the alarmone ppGpp. J. Bacteriol. 187:5460-5469 -- PubMed -- PDF

Dombrecht B., Heusdens C., Beullens S., Verreth C., Mulkers E., Proost P., Vanderleyden J., Michiels J. (2005) Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin. Mol. Microbiol. 55:1207-1221 -- PubMed -- PDF

Daniels R., Vanderleyden J., Michiels J. (2004) Quorum sensing and swarming migration in bacteria. FEMS Microbiol. Rev. 28:261-289 -- PubMed -- PDF

Dombrecht B., Marchal K., Vanderleyden J., Michiels J. (2002) Prediction and overview of the RpoN-regulon in closely related species of the Rhizobiales. Genome Biol. 3:0076.1-0076.11 -- PubMed -- PDF

Michiels J., Xi C., Verhaert J., Vanderleyden J. (2002) Functions of Ca2+ in bacteria. A role for EF-hand proteins? Trends Microbiol. 10:87-93 -- PubMed -- PDF

Daniels R., De Vos D.E., Desair J., Raedschelders G., Luyten E., Rosemeyer V., Verreth C., Schoeters E., Vanderleyden J., Michiels J. (2002) The cin quorum-sensing locus of Rhizobium etli CNPAF512 affects growth and symbiotic nitrogen fixation. J. Biol. Chem. 277:462-468 -- PubMed -- PDF

Michiels J., Dirix G., Vanderleyden J., Xi C. (2001) Processing and export of peptide pheromones and bacteriocins in Gram-negative species. Trends Microbiol. 9:164-168 -- PubMed -- PDF

Dombrecht B., Vanderleyden J., Michiels J. (2001) Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in Gram-negative bacteria. Mol. Plant-Microbe Interact. 14:426-430 -- PubMed -- PDF

Xi C., Schoeters E., Vanderleyden J., Michiels J. (2000) Symbiosis-specific expression of Rhizobium etli casA encoding a secreted calmodulin-related protein. Proc. Natl. Acad. Sci. USA. 97:11114-11119 -- PubMed -- PDF

Michiels J., Dombrecht B., Vermeiren N., Vanderleyden J. (1998) Phaseolus vulgaris is a non-selective host for nodulation. FEMS Microbiol. Ecol. 26:193-205 -- Publisher -- PDF

Michiels J., Moris M., Dombrecht B., Verreth C., Vanderleyden J. (1998) Differential regulation of Rhizobium etli rpoN2 gene expression during symbiosis and free-living growth. J. Bacteriol. 180:3620-3628 -- PubMed -- PDF

Michiels J., Van Soom T., D’hooghe I., Dombrecht B., Benhassine T., de Wilde P., Vanderleyden J. (1998) The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN, ptsN and ptsA mutants. J. Bacterio. 180:1729-1740 -- PubMed -- PDF

Rosemeyer V., Michiels J., Verreth C., Desair J., Vanderleyden J. (1998) luxI- and luxR-homologous genes of Rhizobium etli CNPAF512 contribute to the synthesis of autoinducer molecules and nodulation of Phaseolus vulgaris. J. Bacteriol. 180:815-821 -- PubMed -- PDF


Other research topics

Swarming behavior of Rhizobium etli and Pseudomonas aeruginosa

Fauvart M., Phillips P., Bachaspatimayum D., Verstraeten N., Fransaer J., Michiels J., Vermant J. (2012) Surface tension gradient control of bacterial swarming in colonies of Pseudomonas aeruginosa. Soft Matter 8:70-76 -- Publisher -- PDF -- Supplementary Movie 1 -- Supplementary Movie 2

Verstraeten N., Braeken K., Debkumari B., Fauvart M., Fransaer J., Vermant J., Michiels J. (2008) Living on a surface: swarming and biofilm formation. Trends Microbiol. 16:496-506 -- PubMed -- PDF

Daniels R., Reynaert S., Hoekstra H., Verreth C., Janssens J., Braeken K., Fauvart M., Beullens S., Heusdens C., Lambrichts I., De Vos D.E., Vanderleyden J., Vermant J., Michiels J. (2006) Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli. Proc. Natl. Acad. Sci. USA. 103:14965-14970 -- PubMed -- PDF 

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